Celloger Pro application: NK cell killing assay

In this video, CURIOSIS demonstrates how the Celloger^® Pro can be used to studying NK cell interactions with target cells. To observe these interactions, U-2OS cells (targets) were stained with CellTracker Green CMFDA (green fluorescence) and co-cultured with NK-92 cells (effectors) at effector-to-target ratios of 5:1 and 20:1. Time-lapse imaging began immediately after staining with 4 µM EthD-1 (red fluorescence) and was performed every hour for 24 hours using a Celloger^® Pro with a 4X objective lens.