Low-Input DNA Methylation Analysis at Emory University

DNA methylation is essential for mammalian development and is often dysregulated in disease. Understanding the dynamics of DNA methylation can thus provide insight into disease etiology and serve as a biomarker. Base-pair resolution analysis of DNA methylation is facilitated by sequencing of bisulfite treated DNA, but this process can be laborious and expensive. Reduced representation bisulfite sequencing (RRBS) uses restriction enzymes to enrich parts of the genome where CpG dinucleotides occur, thus decreasing sequencing costs. In this video presentation from ASHG 2015, Ben Berwick of Emory University presents a robust methodology for RRBS analysis using methods from Kapa Biosystems.