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AAV NAb Assays

Svar Life Science ABAvailable: Worldwide

A two-component system of an AAV serotype-producing cell and a iLite®  responsive reporter cell that accurately provides a semi-quantitative NAb assay. The luciferase reporter gene expresses proportionally to the AAV transduction level, enabling precise detection of neutralizing antibodies against AAV. AAV-producing cells are available for serotypes 2, 5, 6, 8, and 9, with customizable options for other serotypes or derivative…

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iLite® AAV Responsive Reporter Assay Ready Cells are based on the human embryonic kidney 293 (HEK293) cell line and are ideal for neutralizing antibody detection studies. Designed to respond to the transduction (interaction, uptake, and nuclear effects) of AAV vectors made with iLite® AAV Packaging Assay Ready Cells.

Features and benefits:

  • Firefly Luciferase expression driven by AAV transduction
    Constitutive Renilla Luciferase expression for internal control:
    Allowing for determining biological sample citotoxyc effect
    Results independent of number of cells
  • iLite® AAVx Packaging Assay Ready Cells are derived from human embryonic kidney 293 (HEK293) cell line.
  • iLite® AAVx Packaging Assay Ready Cells are ideal for neutralizing antibody detection studies. Engineered and optimized to produce AAV vectors of serotype 2,5,6,8 or 9.

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