AAV2 Titration ELISA
Unique microtiter plate enzyme immunoassay for the quantitation of virions and assembled empty capsids of human Adeno-associated Virus 2. The capture-antibody detects a conformational epitope not present on unassembled capsid proteins. The assay represents a fast, sensitive and reproducible method for titration of intact AAV2 wt virions, AAV2 recombinant virions or assembled and intact empty AAV2 capsids. The A20 antibody is…
Standard curve example
Analysis of matrix effects of additives
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Very nice product.
Analysis
It is easy to use and good quality, nice product.
Review Date: 27 Sept 2021 | PROGEN
Great kit, it is part of our QC ELISA assays, highly recommended.
Biotechnology, quality control
It's quite a trustworthy ELISA kit, you get good performance in the assay and robust results. It's quite handy as all reagents, including controls, come with the kit.
Review Date: 24 Dec 2020 | PROGEN
Truly the best ELISA.
Viral vectors
I love the Progen titration ELISA. They are easy to use and give reliable, fast results. With the new Xpress ELISAs, they are even faster. Overall, I am a very happy customer.
Review Date: 8 Dec 2020 | PROGEN
Excellent quality; our analytical method of choice to compare with other analytics.
Analyze capsid titer in sample
This kit is easy to use and the results are robust and reproducible. The quality control of the kit is excellent and yields results that are important to assessing the capsid quality in samples from bioreactors and other samples. Technical service is always knowledgeable and helpful with any questions or troubleshooting.
Review Date: 8 Dec 2020 | PROGEN
Consistent results and easy to use.
Quantification of viral particles
Ready to use ELISA kit for the titration of assembled AAV capsid particles that yields consistent and reliable titres across different samples and concentrations.
Review Date: 12 Feb 2020 | PROGEN
Fantastic product which is a core component of our R&D work into gene therapy.
R&D into the production of rAAV for gene therapy. We are using this ~3 times a week and are very hap
We use this product ~3 time per week and receive very reliable, consistent results; it is a critical part of our development within R&D that we cannot perform our work without. Alongside the AAV2 kit, we also use the AAV5 (PRAAV5) and AAV9 (PRAAV9) ELISA kits which we are also extremely satisfied with. Our after purchase care has also been exceptional with fast replies to any queries and opportunities to meet with the team.
Review Date: 17 Dec 2018 | PROGEN
The ELISA principle:
The assay is based on the sandwich ELISA technique where a monoclonal antibody (mab) specific for a conformational epitope on assembled AAV capsids is coated onto the plate and is used to capture AAV particles from the specimen.
The detection of captured AAV particles is a two-step process.
A biotin-conjugated mab is bound to the captured AAV particles.
A streptavidin peroxidase conjugate reacts with the biotin molecules. The addition of the substrate results in a color reaction which is proportional to the amount of specifically bound viral particles.
Comparison of AAV Quantification Methods:
Each of the commonly used quantification methods has its pros and cons:
- qPCR is widely used, but suffers from several issues such as sample preparation, primer design, or PCR efficiency that can lead to high inter-laboratory variation of results.
- Digital droplet PCR methods overcome some of the limitations of qPCR. However, variations between labs can still occur due to different sample processing protocols.
- Dot blot is a simple and quantitative method, if reliable reference material is used. However, it suffers from the limited linearity and dynamic range of western blotting in general.
Given the practical drawbacks of the aforementioned techniques, a conventional sandwich ELISA currently appears to be superior in terms of inter- and intralaboratory variation as well as ease of use. Therefore, it represents the best format for reliable and reproducible quantification of total rAVV capsid titers.
Use of shuffled/mutated AAV:
The recognition of shuffled/mutated AAV vectors depend on the specific capsid region which is affected by the shuffling/mutation. The capture antibodies used for PROGEN's AAV ELISAs bind specific and, in some cases, well defined conformational epitopes. These epitopes are generated by the capsid assembly of the corresponding AAV serotypes. A first indication that the ELISA might recognize your shuffled/mutated AAV vector is the presence of the antibody-binding epitope. However, changes in the protein sequences of the capsid proteins might also influence conformation of the proteins, hence the conformation of the epitopes presented on the AAV capsid. This might influence binding affinity of the antibody and affect determination of the titer based on the (non-shuffled) Kit Control provided with the AAV ELISA kit. Since these properties strongly depend on the specific shuffling/mutation performed, PROGEN cannot guarantee successful and precise quantification of your shuffled/mutated AAV vector. Even if the antibody binding epitopes are still present on your shuffled/mutated AAV capsid, your assay needs to be tested and optimized for your specific AAV vector.PR
OGEN highly recommends the production and calibration of a suitable (shuffled/mutated) Kit Control, to ensure reliable titer determination of your individually shuffled/mutated AAV vector with PROGEN ELISA kits.
Limited Use Label License: Research Use OnlyProduct is exclusively licensed to PROGEN Biotechnik GmbH. The use of these products for the development, manufacturing and sale of secondary products/derivativeswhich are based on the purchased products and/or which include the purchased product require a royalty based sub-license agreement.
