AAV2 VP1 + VP2 + VP3, recombinant proteins, set
N-terminal His-tagged recombinant AAV2 capsid proteins VP1 + VP2 + VP3.
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The AAV capsid consists of three capsid proteins, i.e. VP1, VP2, and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
This set of recombinant AAV2 VP1, VP2, and VP3 can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV2 capsid proteins are available as a set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).
Note: please find an example of how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
