AAV2 VP3, recombinant protein
N-terminal His-tagged recombinant AAV2 capsid protein VP3
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The AAV capsid consists of three capsid proteins, i.e. VP1, VP2 and VP3, which differ in their N-terminus and encapsulate the genomic ssDNA. In native virus particles, the three proteins form subunits with a ratio of 1:1:10 (VP1:VP2:VP3), in a total number of 60 subunits per capsid.
The recombinant AAV2 VP3 protein in combination with recombinant AAV2 VP1 (Cat. No. 640823) and recombinant AAV2 VP2 (Cat. No. 640824) can be used to create a mixture with the precise molar ratio of 1:1:10 to compare the protein composition of the viral capsid in your sample by protein detection methods, e.g. western blot.
All three recombinant AAV2 capsid proteins are available as a set (Cat. No. 72001) or as individual proteins (Cat. No. 640823, 640824, 640825).
Note: please find an example of how to prepare western blot samples in the pipetting scheme below. Aliquots of the remaining samples can be stored at -80°C for reuse.
