AlphaLISA
Alpha is a bead-based, no-wash luminescent technology. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions is set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events, offering maximum flexibility.
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IL6 and IL10 quantification
We used the IL6 and IL10 AlphaLISA kits for a medium throughput screen and found the assays to be fast, easy and highly reproducible.
Review Date: 16 Jan 2015 | Revvity
Alpha technology is compatible with a wide range of sample types ranging from simple buffers, cell supernatants, and cell lysates to complex tissue, serum, plasma, and other biological samples.
Alpha offers benefits including:
- Faster time to results
- Ability to quantitate large protein complexes, nucleosomes, and viral particles
- Option for multiplexing
Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.
- Ease-of-use: homogeneous assay with no wash requirement and no separation steps
- Fast: results in 3 hours for most applications
- High sensitivity: detect down to femtogram levels of target
- Large dynamic range: 4-5 logs
- Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more
Choose the homogenous assay right for you:
- AlphaLISA® – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
- AlphaLISA SureFire®Ultra™ – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
- AlphaScreen® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
- Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own
