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Annexin V IgG/IgM, anti-

DRG International Inc.EIA-3971Available: Worldwide

High Quality Assays with Reproducible and Reliable Results

DRG International Inc.

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Anti-Annexin V IgG/IgM ELISA is a test system for the quantitative measurement of IgG and IgM class autoantibodies against Annexin V in human serum or plasma.

This product is intended for professional in vitro diagnostic use only.

Highly purified Annexin V is bound to microwells. The determination is based on an indirect enzyme linked immune reaction with the following steps:

  • Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells.
  • After incubation, a washing step removes unbound and unspecifically bound serum or plasma components.
  • Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes.
  • After incubation, a second washing step removes unbound enzyme conjugate.
  • After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue colored product.
  • Addition of an acid stops the reaction generating a yellow end-product.
  • The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm.

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