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Annexin V IgG/IgM, anti-
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Anti-Annexin V IgG/IgM ELISA is a test system for the quantitative measurement of IgG and IgM class autoantibodies against Annexin V in human serum or plasma.
This product is intended for professional in vitro diagnostic use only.
Highly purified Annexin V is bound to microwells. The determination is based on an indirect enzyme linked immune reaction with the following steps:
- Specific antibodies in the patient sample bind to the antigen coated on the surface of the reaction wells.
- After incubation, a washing step removes unbound and unspecifically bound serum or plasma components.
- Subsequently added enzyme conjugate binds to the immobilized antibody-antigen-complexes.
- After incubation, a second washing step removes unbound enzyme conjugate.
- After addition of substrate solution the bound enzyme conjugate hydrolyses the substrate forming a blue colored product.
- Addition of an acid stops the reaction generating a yellow end-product.
- The intensity of the yellow color correlates with the concentration of the antibody-antigen-complex and can be measured photometrically at 450 nm.
