ApopTag® Fluorescein In Situ Apoptosis Detection Kit
Detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that's conjugated to a Fluorescein reporter molecule
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
TUNEL Assay live NP
This kit provides all the necessary means to perform a TUNEL assay. We utilize this on fixed tissue and it works exceptionally well.
Review Date: 15 Nov 2017 | Merck
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit detects apoptotic cells in situ by the indirect TUNEL method, utilizing an anti-digoxigenin antibody that is conjugated to a Fluorescein reporter molecule. It provides indirect immunofluorescence staining for 40 samples. Results are analyzed using either flow cytometry or fluorescence microscopy.
The ApopTag® Fluorescein In Situ Apoptosis Detection Kit has been tested for specific staining in these model systems: (a) human normal peripheral blood lymphocytes induced with dexamethasone as stained in cytospins, (b) rat regressing mammary gland as stained in formalin-fixed, paraffin-embedded sections, and (c) human leukemic peripheral blood lymphocytes induced with camptothecin, as stained in cell suspensions and used for quantitative flow cytometry.
Components:
- Equilibration Buffer 90416 3.0 mL -15°C to -25°C
- Reaction Buffer 90417 2.0 mL -15°C to -25°C
- TdT Enzyme 90418 0.64 mL -15°C to -25°C
- Stop/Wash Buffer 90419 20 mL -15°C to -25°C
- Blocking Solution 90425 2.6 mL -15°C to -25°C
- Anti-Digoxigenin-Fluorescein 90426 2.1 mL 2°C to 8°C
- Plastic Coverslips 90421 100 ea. Room Temp.
- affinity purified sheep polyclonal antibody