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Cellartis® Human iPS cells

Cellartis Human iPS Cell Line Kits

Takara Bio

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Average Rating 3.7

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Average Rating 3.7

Application Area:

Pluripotent stem cell research

Cellartis human pluripotent stem cell kits are a great way to introduce new users to pluripotent stem cells, as normally these cells require not only previuos training in cell culture, but a period of a year to get to be able to generate these cells. Not the case with these cells, as they include media and extracelular matrix along to start culture in a ready to use manner good value for money, they require a little care after thawed.

Review Date: 8 Feb 2021 | Takara Bio

Cellartis® Human iPS Cell Line Kits contain human induced pluripotent stem (iPS) cells that are banked and characterized according to the highest industry standards, along with a starter set for the Cellartis DEF-CS Culture System.

The iPS cells are adapted to the Cellartis DEF-CS Culture System, an easy-to-use, complete culture system for efficient iPS-cell expansion in a feeder-free, defined environment. With this system, cells are maintained in an undifferentiated state with virtually no background differentiation, eliminating the need for cell selection. The highly reproducible nature of the DEF-CS system coupled with the stable high growth rate of these stem cell lines is ideal for mass production of cells.

Features:

  • Cell lines were created by reprogramming human skin fibroblasts using defective polycistronic retrovirus technology to deliver OCT-4, SOX2, KLF4, and c-Myc
  • Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (OCT-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin, FOXA2, ASMA), and expected karyotype
  • Optimized for culture with the Cellartis DEF-CS Culture System
  • Suitable for all major applications; cells can form derivatives of all three germ layers when differentiated
  • Kits include the Cellartis DEF-CS 100 Culture System

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