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CellTrace™ CFSE Cell Proliferation Kit

Thermo Fisher ScientificC34554View all modelsAvailable: Worldwide

This product is used for labeling of cells to trace multiple generations by flow cytometry.

Thermo Fisher Scientific

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Average Rating 5.0

|2Scientists have reviewed this product

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Clear results, easy to use.

 

Average Rating 5.0

Application Area:

Immunology T cell proliferation

Very effective labeling and low toxicity.

Review Date: 26 Sept 2023 | Thermo Fisher Scientific

Great cell staining, will consistently use in future.

 

Average Rating 5.0

Application Area:

Tracking cell proliferation

The CellTrace CFSE proliferation kit is extremely reliable and effective for detecting proliferation of cells in vitro and in vivo. It has great value for money, with easy to follow instructions for consistent staining of cells.

Review Date: 16 Jul 2020 | Thermo Fisher Scientific

CellTrace™ CFSE Cell Proliferation Kit (Ex/Em 492⁄517 nm) is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

  • Superior performance—bright, single-peak staining enables visualization of multiple generations
  • Long-term signal stability—well-retained in cells for several days post stain
  • Simple, robust staining protocol


Successful proliferation analysis by dye dilution requires an extremely bright dye to distinguish fluorescently labeled cells from auto-fluorescence after several cell divisions. The intense fluorescent staining provided by CellTrace™ CFSE dye enables the visualization of eight or more generations of proliferating cells before the signal is overwhelmed by intrinsic cellular auto-fluorescence. Consistent, homogeneous staining results in very little fluorescence variation between cells in a population, so distinct generations can be seen without any requirement for complex modeling software.

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