CellTrace™ Dyes for Cell Proliferation

Permanently label cells with Molecular Probes™ CellTrace™ fluorescent stains without affecting morphology or physiology to trace generations or divisions in vivo or in vitro.

The CellTrace™ Cell Proliferation Kits provide versatile and well-retained cell tracing reagents in a convenient and easy-to-use form. Each kit contains a CellTrace™ reagent in single-use vials to permit small scale experiments without preparing excess quantities of stock solution. The CellTrace™ reagents readily diffuse into cells and bind covalently to intracellular amines, resulting in stable, well-retained fluorescent staining that can be fixed with aldehyde fixatives. Excess unconjugated reagent passively diffuses to the extracellular medium, where it can be quenched with complete media and washed away.

Features & Benefits:

• Superior performance—bright, single-peak staining enables visualization of multiple generations
• Long-term signal stability—well retained in cells for several days post-stain
• Non-cytotoxic—no known effect on proliferative ability or biology of cells
• Versatile—multiple colors available to easily combine with antibodies or markers of cell function, such as GFP

CellTrace Cell Proliferation Kit selection:

• CellTrace™ CSFE (Cat. no. C34554)
  The approximate excitation and emission peaks of this product after hydrolysis are 492 nm and 517 nm, respectively. Cells labeled with CellTrace™ CSFE reagent can be visualized by fluorescence microscopy using standard fluorescein filter sets or analyzed by flow cytometry in an instrument equipped with a 488-nm excitation source.
  
• CellTrace™ Violet (Cat. no. C34557)
  The approximate excitation and emission peaks of this product after hydrolysis are 405 nm and 450 nm, respectively. Cells labeled with CellTrace™ Violet reagent can be visualized by fluorescence microscopy using standard DAPI filter sets or analyzed by flow cytometry in an instrument equipped with a 405-nm excitation source.
  
• CellTrace™ Far Red (Cat. no. C34564)
  The approximate excitation and emission peaks of this product after hydrolysis are 630 nm and 661 nm, respectively. Cells labeled with CellTrace™ Far Red reagent can be visualized by fluorescence microscopy using standard Cy™ 5 filter sets or analyzed by flow cytometry in an instrument equipped with a 633/635-nm excitation source.
  
• CellTrace™ Yellow (Cat. no. C34567)
  The approximate excitation and emission peaks of this product after hydrolysis are 555 nm and 580 nm, respectively. Cells labeled with CellTrace™ Yellow reagent can be visualized by fluorescence microscopy using the standard FITC filter sets or analyzed by flow cytometry in an instrument equipped with a 532-nm or 561-nm excitation source.