Products & ReviewLife Sciences

Corticosterone ELISA

DRG International Inc.EIA-4164Available: Worldwide

High Quality Assays with Reproducible and Reliable Results

DRG International Inc.

The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our  Product Directory.

Average Rating 5.0

|1Scientists have reviewed this product

Ease of Use
After Sales Service
Value for Money
Write your own review

Average Rating 5.0

Application Area:

Rat and mouse blood-plasma

Amount of standard 0 provided with the kit was too low, but DRG immediately sent some more. Everything else worked fine and we get good results.

Review Date: 30 Jan 2015 | DRG International Inc.

An enzyme immunoassay for the quantitative measurement of corticosterone in serum and plasma. Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone is a major indicator of stress since stress increases the production of corticosteroids. Studies involving corticosterone and levels of stress include impairment of long term memory retrieval1, chronic corticosterone elevation due to dietary restrictions2 and in response to burn injuries3. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns 4,5.The DRG Corticosterone ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA), based on the principle of competitive binding. The microtiter wells are coated with a polyclonal antibody directed towards an antigenic site on the Corticosterone molecule. Endogenous Corticosterone of a patient sample competes with a Corticosterone-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is reverseproportional to the concentration of Corticosterone in the sample. After addition of the substrate solution, the intensity of colour developed is reverse proportional to the concentration of Corticosterone in the patient sample.

Product Overview

Links