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CRISPR & shRNA Lentiviral Packaging Mix

This Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.

Merck

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Lentiviral particles are generated from three main components:

• The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.

• The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells

• The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.

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