CRISPR Human Genome 80K Knockout Library (plasmid)
Cellecta’s 80K Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules. With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we hav…
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High titer makes consistency and penetration of difficult cell models better.
Functional Genomics - lentiviral arrayed CRISPR libraries for target discovery
Because of high titer, result consistency and reagent delivery it is great. Particularly for physiologically relevant cell models which we are trying to enable for more target identification.
Review Date: 28 Jan 2020 | Cellecta
Cellecta’s 80K Single-module Human Genome-Wide CRISPR Library was designed to be small enough (~80,000 constructs) to enable convenient genome-wide “dropout viability” screens with most standard human cell modules.
With 4 sgRNAs targeting each gene, all of which have been designed using the latest criteria for effective guides described by Doench, et al. and containing the Cellecta-designed HEAT sgRNA structure which we have proven to generate more robust screening results than the standard sgRNA design, the library has deep enough gene coverage to generate efficient results.
Genome-Wide Coverage with a Convenient Single 80K Module
- Single library with ~80,000 constructs targeting approximately 19,000 protein-coding genes
- Each gene is targeted by 4 sgRNAs designed based on Doench, et al. criteria
- Targets all human protein-coding genes—over 19,000 gene targets.
The 80K Single-Module Human Genome-Wide CRISPR Library includes the following controls
- standard non-targeting controls
- non-specific control (sgRNA targeting introns throughout the genome)
- lethal sgRNA targeting generally essential genes (i.e., positive controls for dropout screens)
