CRISPR Universal Negative Control
Universal negative control CRISPRs have been designed not to recognize any sequence in the human, mouse or rat genome.
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A single vector format is provided which includes the Cas9 expression cassette and gRNA sequence. This vector includes GFP which is co-expressed from the same mRNA as the Cas9 protein via a 2A peptide linkage and enables for tracking of transfection efficiency or enrichment in cell populations via FACS.
Application
• Creation of gene knockouts in cell lines
• Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
• Allows for expression of Cas9 and GFP without specific targeting of the CRISPR/Cas9 system.
