DreamTaq™ Hot Start PCR Master Mix
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes. DreamTa…
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Quick and easy to use.
DNA amplification
DreamTaq™ Hot Start Green PCR Master Mix is an all in one master mix, easy and quick to use and the PCR product is well detected.
Review Date: 17 Jul 2021 | Thermo Fisher Scientific
Easy to use, good quality amplification
Routine qualitative PCR
The hot start master mix takes all the pain out of PCR. Super simple. Great amplification and fidelity for a relatively inexpensive polymerase.
Review Date: 28 Jun 2021 | Thermo Fisher Scientific
Accurate results, trustworthy, work-efficient.
Analyzing heptatisis virus in blood plasma
It is an excellent product that produces accurate and precise results in clinical labs as well as in research labs.
Review Date: 8 Jun 2020 | Thermo Fisher Scientific
Great Master Mix with clear specific bands, no genomic contamination.
PCR, Genotyping
Great Master Mix with clear specific bands, no genomic contamination... compared to other reagents, DreamTaq looks to be highly specific with minimal genomic DNA contamination.
Review Date: 27 Feb 2019 | Thermo Fisher Scientific
I've used a free sample and I get results!
DNA Amplification
Very easy to use the hot start master mix! I've successes in three of four DNA Amplifications. The length of PCR product more than 5000cb in length.
Review Date: 20 Mar 2018 | Thermo Fisher Scientific
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes.
DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases. DreamTaq Hot Start DNA Polymerase employs antibody-based inhibition of DNA polymerase activity at ambient temperatures to prevent the amplification of non-specific products prior to the amplification step. With DreamTaq Hot Start DNA Polymerase, reactions can be set up at room temperature using the same protocol and cycling conditions as conventional Taq DNA polymerases.
DreamTaq Hot Start PCR Master Mix is formulated to enhance productivity:
• Better reaction outcomes
--Higher yield of target amplicons from low template amounts
--Increased reaction specificity due to reliable hot-start technology
--Wider range of amplicon lengths - routine amplification of genomic DNA fragments up to 6kb
• Enhanced convenience
--Room temperature reaction set-up
--Minimized optimization of Mg2+ concentration and of primer annealing temperatures due to optimized reaction buffer
--Simplified workflow and minimized pipetting steps with 2X master mix format
Applications:
• Routine PCR
• Colony PCR
• Genotyping
• RT-PCR
• Generation of PCR products for TA cloning
