EnGen sgRNA Synthesis Kit
The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.
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sgRNA made easy and cheap by NEB.
sgRNA synthesis for CRISPR
The NEB kit for making one's own sgRNA is easy to use. Makes 17ug and only takes 30 minutes. All you have to supply is a 50nt DNA guide sequence. For $250 and ten uses, this kit is very affordable compared to ordering synthetic RNA kits. This kit is another home run for NEB.
Review Date: 23 Jul 2020 | New England Biolabs Inc.
The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.
- Rapid generation of microgram quantities of sgRNA
- Complete workflow of less than one hour
- Simple protocol requires only a user-supplied ~55 nucleotide ssDNA target-specific oligonucleotide
- For help with oligo design, try EnGen sgRNA Template Oligo Designer - now included in the NEBioCalculator® Tool
- Compatible with EnGen Spy Cas9 NLS, EnGen Spy Cas9 Nickase, EnGen Spy dCas9 (SNAP-tag®)
