ExoBrite™ EV Total RNA Isolation Kit
Optimized and easy-to-use kit for extracting total RNA, including mRNA and miRNA, from purified EVs for downstream analysis in qPCR or RNAseq.
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Obtaining high yields of quality RNA from extracellular vesicles (EVs) is a challenge due the dilute nature of EV samples and low RNA content within EVs. In addition, short non-coding RNAs within EVs, such as miRNAs, are commonly studied as biomarkers and often require specialized methods for efficient extraction. The ExoBrite™ EV Total RNA Isolation Kit was designed to address these challenges by offering an optimized and easy-to-use kit for total RNA isolation, including mRNA and miRNA, from purified EVs. The isolated EV RNA can then be used for downstream analysis such as qPCR or RNAseq.
Features
- Extract total RNA from purified EVs
- Recover ~10-20 ng of RNA from 1×1010 SEC-enriched EVs
- Compatible with downstream applications such as qPCR or RNAseq
- Simple column-based purification
- No phenol/chloroform or ethanol precipitation steps
The RNA isolation procedure is a simple column purification method that takes as little as 20 minutes and requires no phenol/chloroform or ethanol precipitation steps. The kit is estimated to recover ~10-20 ng of RNA from 1×1010 SEC-enriched EVs. The amount of RNA recovered will depend primarily on EV number and quality, which may be affected by purification method, storage conditions, and number of freeze thaws. An optional, but recommended, DNase treatment step is used to remove contaminating DNA.
