F(ab')2 Fragment Affinity-Purified Secondary Antibodies
F(ab')2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region. Used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G.
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Gives great results!
Remove Fc region
I used the product and it gives accurate results, along with the ease of using it. So far, I am really happy with my purchase and would recommend it to other researchers.
Review Date: 27 Jul 2021 | Jackson ImmunoResearch Laboratories Inc.
Needs to improve efficiency
Protein protein interactions
Products aren’t working sometimes. After sales service needs to improved
Review Date: 2 Jul 2020 | Jackson ImmunoResearch Laboratories Inc.
Specific.
Western blot
I have used thes antibody to develop a western blot. They are very specific and give no background noise.
Review Date: 1 Jul 2020 | Jackson ImmunoResearch Laboratories Inc.
Great results
Immunohistochemistry
Easy to use, inexpensive, high quality and reproduce with low background
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Great antibodies!
Anylyze transcription factors in tissue samples
It will be better if pre-dissolving the 2nd antibodies in 50% glycerol.
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Reliable antibody
Flow cytometry measurement on cell surface marker detection
This is a reliable product. We can reproduce dependable results with it. We had no issue with it.
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Good and reliable results
analyzed circulating metabolites in serum samples
Easy to use - high quality and specificity - no non-specific false results.
Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.
Application: Double or triple labeling immunofluorescence
As long as the host species for the primary antibodies are something different, the Jackson's secondary antibodies give excellent results with no cross reactivity between them.
Review Date: 28 Jul 2015 | Jackson ImmunoResearch Laboratories Inc.
F(ab')2 fragments of antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving intact some of the hinge region.
Used for specific applications, such as to avoid binding of antibodies to live cells with Fc receptors or to Protein A or Protein G.
