Fab Fragment Affinity-Purified Secondary Antibodies
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. Block endogenous immunoglobulins on cells, tissues , or other surfaces. Block exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species. Fab-label primary antibodies in solution without compromising activity.
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Works well at high concentrations
Immunohistochemistry
Very useful for an immunohistochemistry with two primary antibodies from the same host. It covers the first primary antibody, presenting it as a different species. Used at a concentration of 40 ug/ml with the following protocol: https://www.jacksonimmuno.com/technical/products/protocols/double-labeling-same-species-primary/example-b
Review Date: 8 Aug 2016 | Jackson ImmunoResearch Laboratories Inc.
Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region.
- Block endogenous immunoglobulins on cells, tissues , or other surfaces.
- Block exposed immunoglobulins in multiple labeling experiments using primary antibodies from the same species.
- Fab-label primary antibodies in solution without compromising activity.
