FirePlex Immunoassays
Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets.
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Abcam's proprietary FirePlex particle technology is ideal for multiplexing protein targets. The bar-coded hydrogel particles allow the bulk immobilization of capture antibody, providing enhanced binding capacity in comparison to surface-functionalization techniques. The flexibility of the hydrogel matrix reduces steric constraints on the binding while enhancing binding kinetics in a planar array format, making assay readouts possible both on flow cytometers as well as High Content Imagers.
FirePlex Immunoassays are offered in three complementary formats for convenient scale-up of samples/compounds for screening:
FirePlex-96 assay:
- Ideal for target screen in 96-well plate format
- pg sensitivity and 5-log dynamic range
- Simple, straight forward workflow
- Readout on a flow cytometer
FirePlex-384 assay:
- Ideal for high throughput screening in 384-well plate format
- Small sample volume of 6.25 µl
- No-wash, two-step - automation friendly
- Readout on High Content Imager
FirePlex-1536 assay:
- Ideal for high throughput screening in 1536-well plate format
- Small sample volume of 2.5 µl
- No-wash, one-step - automation recommended
- Readout on High Content Imager
