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FLAG® Immunoprecipitation Kit

Sigma-Aldrich SupelcoAvailable: Worldwide

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. 

Sigma-Aldrich Supelco

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Average Rating 4.0

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Very efficient. We use 2 times more FLAG peptide than what's recommended on datasheet

 

Average Rating 4.0

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FLAG Immunoprecipitation

Very efficient. We use 2 times more FLAG peptide than what's recommended on the datasheet. This significantly helped us improve the yield of eluates without interfering with Mass Spectrometry and not giving background. It's a pity that some components within the kit cannot be bought separately.

Review Date: 31 Mar 2022 | Sigma-Aldrich Supelco

The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin under acidic conditions or by competition with FLAG peptides. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Application

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

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