Flow Cytometer Calibration Beads

Flow Cytometer Calibration Beads for AcGFP/EGFP and mCherry.

Use these beads to calibrate your flow cytometer prior to analyzing cells that express the AcGFP1 or mCherry fluorescent proteins. The AcGFP Flow Cytometer Calibration Beads allow for easy calibration of any flow cytometer with a 488 nm laser line that excites the green fluorescent proteins AcGFP1 (Aequorea coerulescens GFP) and EGFP. [The AcGFP Flow Cytometer Calibration Beads work for both AcGFP1 and EGFP because their excitation/emission spectra and brightness are almost identical.] The mCherry Flow Cytometer Calibration Beads allow for easy calibration of any flow cytometer with a 561 nm laser line that excites the red fluorescent protein, mCherry.


Features:

• The lowest intensity represents the autoflorescence signal of cells not expressing the fluorescent protein (AcGFP1 or mCherry)
• The remaining five peaks are evenly distributed over the remaining scale of the green or red fluorescence detection channel
• Works with any flow cytometer with a 488 nm laser line (AcGFP1) or a 561 nm laser line (mCherry)

Applications:

• Calibrate your flow cytometer prior to analyzing cells expressing AcGFP1 or mCherry fluorescent protein
• Since the spectral properties and brightness of AcGFP1 and EGFP are very similar, these beads may be used to calibrate flow cytometers prior to using EGFP expressing cells