GIPZ Lentiviral shRNAmir
shRNAmir-GFP: Visualize RNAi Thermo Scientific GIPZ Lentiviral shRNAmir advantages: shRNAmir design for specificity and increased knockdown efficiency TurboGFP marks cells expressing shRNAmir Efficient low copy knockdown- Important for pooled screens Genome-wide human and mouse coverage Perform efficient RNAi in primary and non-dividing cells Guaranteed* knockdown Please note that the pGIPZ and pTRIPZ vectors a…
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I have had the best experience with open biosystems. Long before I even ordered, I was in contact with a person in Open Biosystems who helped me search my gene, answered my questions and answered my emails sometimes within the hour. We decided to go with the high-titer virus instead of making them ourselves. They gave us a quote and the quote which I got directly from Open Biosystem was 10 times cheaper (not exactly sure why) than from Thermo directly. But anyway, the virus worked great. I got 99% transfection efficiency and 90% knockdown of my protein. I love this product and would recommend it to all. I used this for b cells (non-adherent cells) and there were no protocols around. But from 23% efficiency at day 3 without puromycin to 99% on day 7. I was set to go within a week.
Review Date: 14 Jul 2011 | Thermo Fisher Scientific
shRNAmir-GFP: Visualize RNAi
Thermo Scientific GIPZ Lentiviral shRNAmir advantages:
- shRNAmir design for specificity and increased knockdown efficiency
- TurboGFP marks cells expressing shRNAmir
- Efficient low copy knockdown- Important for pooled screens
- Genome-wide human and mouse coverage
- Perform efficient RNAi in primary and non-dividing cells
- Guaranteed* knockdown
Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen.
*When used in GIPZ RNAintro kits according to kit instructions.
