High-Fidelity PCR Kits
High-fidelity PCR reagents featuring the thermostable iProof Polymerase. This enzyme utilizes Sso7d fusion polymerase technology to provide highly accurate amplification of targets up to 37 kb.
PCR Master Mixes and Kits
Place your order directly with the manufacturer.
Worth the price.
DNA analysis
DNA fragments that other enzymes could not amplify may be amplified by using this enzyme. For this reason, I always keep a tube of this enzyme in my laboratory.
Review Date: 22 Jun 2021 | Bio-Rad
iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.
Features and Benefits of iProof High-Fidelity DNA Polymerase:
- High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
- Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
- Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)
Choose the best DNA polymerase for your application.
Related Categories
- PCR Core Reagents (PCR Buffers & Reagents)
- RNA Isolation Kits
- Reverse Transcription Kits
- Real-time PCR Supermixes
- PrimePCR Wet-Lab Validated qPCR Assays (available in the U.S., Canada, and EU only)
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Products Model Information
iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl
Bio-Rad100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
iProof™ High-Fidelity DNA Polymerase, 500 U (2 U/µl), 250 µl
Bio-Rad500 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
iProof™ HF Master Mix, 100 x 50 µl rxns, 2.5 ml
Bio-Rad100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for most templates
iProof™ High-Fidelity PCR Kit, 200 U (2 U/µl), 100 µl
Bio-Rad200 U, 2 U/µl, iProof DNA polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, ? DNA, 1.3 and 10 kb primers, DNA standard
iProof™ GC Master Mix, 100 x 50 µl rxns, 2.5 ml
Bio-Rad100 x 50 µl reactions, premixed PCR reagents, includes 2x master mix (0.04 U/µl), DMSO; suitable for GC-rich templates
iProof™ High-Fidelity PCR Kit, 50 U (2 U/µl), 25 µl
Bio-Rad50 U, 2 U/µl, iProof polymerase PCR kit includes 5x reaction buffers, MgCl2 solution, DMSO, dNTPs, ? DNA, 1.3 and 10 kb primers, DNA standard