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iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl

Bio-Rad1725301View all modelsAvailable: Worldwide

100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO

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Average Rating 3.7

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Average Rating 3.7

Application Area:

DNA analysis

DNA fragments that other enzymes could not amplify may be amplified by using this enzyme. For this reason, I always keep a tube of this enzyme in my laboratory.

Review Date: 22 Jun 2021 | Bio-Rad

iProof High-Fidelity DNA Polymerase is a unique Pyrococcus-like proofreading enzyme fused to the Sso7d dsDNA-binding protein to create a thermostable fusion polymerase that accurately amplifies long products from a variety of DNA templates.

Features and Benefits of iProof High-Fidelity DNA Polymerase:

  • High fidelity — iProof DNA polymerase is 52-fold more accurate than Taq polymerase
  • Speed — high processivity dramatically reduces extension (15–30 sec/kb) and overall reaction times
  • Successful amplification of long products with higher yields — fragments up to 37 kb are amplified in less time and with less enzyme (0.25–1 unit/reaction)

iProof Polymerase is available in an easy-to-use master mix and a PCR kit complete with controls.


Related Categories

  • Standard PCR Enzymes and Reagents

  • RNA Isolation Kits

  • Reverse Transcription Kits

  • Real-time PCR Supermixes

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