His-Spin™ Protein Miniprep Kit
Fast His-tagged protein purification.
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Purify extracted proteins
This product is really easy to use. I use it as a final step in my protein synthesis pipeline and it takes no time at all. It has given me really great results that have been checked with SDS-PAGE analysis. I have also been able to replicate the results multiple times. Overall it is a great product I would recommend to others.
Review Date: 24 Nov 2022 | Zymo Research
Protein purification
The product works as described. Easy to follow protocol (easier than most!). Storage is simple and seems to not affect efficacy. I DO recommend.
Review Date: 16 Nov 2021 | Zymo Research
Protein purification in cyanobacteria
This kit is awesome!
Review Date: 19 Mar 2021 | Zymo Research
Protein purification
The product enables simple and fast His-Tag purification for small volumes. For the purification of proteins produced within our cell-free expression system the kit is a valuable tool.
Review Date: 11 Dec 2019 | Zymo Research
Recombinant protein purification
Product is very easy to use, purification is done quickly, protein was very pure and active afterwards.
Review Date: 5 Jun 2019 | Zymo Research
The His-Spin Protein Miniprep provides researchers with a method for fast His-tagged protein purification. The easy-to-follow procedure is based on a nickel-charged His-Affinity Gel (IMAC), innovative protein purification, and unique Zymo-Spin Column technology. Up to 1 mg of His-tagged protein can be purified in as little as 5 minutes and can be eluted into as little as 100 µl of the provided His-Elution Buffer. The purified protein can be used directly for enzymatic assays, protein biochemical analyses, SDS PAGE, as well as other protein based applications. The His-Spin Protein Miniprep has been optimized to yield maximal protein purity indices: a single protein band is often visualized following Coomassie Blue staining of proteins in SDS-PAGE gel (see figure below). The straightforward spin-wash-elute protocol dramatically simplifies protein purification and results are obtained in minutes, not hours!