Human Complement C3 ELISA Kit (ab108822)
Human Complement C3 ELISA kit is a competitive immunoassay designed for the quantitative measurement of Complement C3 in human plasma and serum.
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Very easy to use, can be adopted even in under-resourced infrastructure settings.
Cancer biomarker discovery
ELISA is highly effective for biomarker discovery due to its sensitivity and specificity. Ease of use is high, but after-sales care varies. Value for money depends on specific needs.
Review Date: 2 Feb 2024 | Abcam plc
A Complement C3 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Complement C3 specific biotinylated detection protein is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Complement C3 captured in plate.
