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In-Fusion® HD Cloning Plus PCR Cloning Kits

Takara Bio6389Available: Worldwide

PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus

Takara Bio

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Average Rating 4.9

|7Scientists have reviewed this product

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Great. I would recommend to everyone who's cloning doesn't work.

 

Average Rating 5.0

Application Area:

Molecular biology

InFusion HD cloning system offers seamless way for rapid plasmid construction. It enables direct cloning of PCR products to linearized vectors without the need of previous purification steps or vector dephosphorylation. Definitely a useful alternative to ligation-dependent cloning.

Review Date: 25 Mar 2022 | Takara Bio

Easy to use

 

Average Rating 5.0

Application Area:

Mutant deletion cloning

Makes design and workload much easier. Really like the online tool.

Review Date: 12 Dec 2017 | Takara Bio

Great cloning strategy

 

Average Rating 5.0

Application Area:

Molecular Biology

This kit turns out to be very easy to use. It saved the cloning of a difficult and long cDNA that we needed and couldn't clone with regular methods.

Review Date: 6 Feb 2017 | Takara Bio

Great results with the In-fusion cloning kit

 

Average Rating 5.0

Application Area:

Plasmid cloning

It was very simple and effective to use. It’s easy to design and clone. It works like a magic mixture.

Review Date: 12 Jul 2016 | Takara Bio

Average Rating 5.0

Application Area:

Cloning

This kit is perfect for cloning larger fragment up to 4kb in first attempt. I am using this kit from last year and it’s perfect for cloning larger construct. The sensitivity is amazing and efficiency of positive clones is above 70%. I highly recommend because of its capability and sensitivity, saved lot of time as compared to normal cloning and decreases repetitive experiment as indirectly save cost as well. When I started using it was new to me as I failed with other cloning product at last I came up with this kit and worked very well since.

Review Date: 13 Oct 2015 | Takara Bio

Average Rating 5.0

Application Area:

Gateway-cloning of BCR constructs

Cloning on the principle of a fire-and-forget system. Fast and easy application in combination with efficiencies of <90% positive clones. Worth to change....

Review Date: 21 May 2015 | Takara Bio

Average Rating 4.3

The best for cloning large sequence inserts.

Review Date: 9 Aug 2008 | Takara Bio

PCR Cloning Kit-Seamless DNA Cloning with In-Fusion HD Cloning Plus

In-Fusion PCR Cloning Kits enable sequence independent, directional, and seamless cloning of ANY PCR fragment-or multiple fragments-into ANY linearized vector at ANY locus in a single 15-minute reaction. No additional treatment of the PCR fragment is required (such as restriction digestion, ligation, phosphorylation, or blunt-end polishing). The efficiency of In-Fusion HD Cloning Kits is over 95% across a broad insert size range (0.5 to 15 kb inserts). The In-Fusion Enzyme premix fuses PCR-generated sequences and linearized vectors efficiently and precisely, utilizing a 15 bp overlap at their ends. This 15 bp overlap can be engineered by designing custom primers for amplification of the desired sequences. This method can be used to clone single or multiple fragments into a single vector without sub-cloning.

In-Fusion HD Plus is a complete cloning solution that includes all the reagents required for successful cloning including the fast and high fidelity CloneAmp HiFi PCR Premix, the ultra-high efficiency Stellar competent cells, and a choice of PCR clean-up or gel extraction kit for DNA purification, along with the In-Fusion HD enzyme premix and reagents for control experiments as standard.

Features:

  • Sub-cloning is unnecessary—Clone any insert, into any location, within any vector
  • Highly efficient—Over 95% efficiency demonstrated with a broad range of fragment sizes from 0.5 kb to 15 kb
  • Seamless construction—Final constructs have no extra base pairs left over (as is often the case with restriction digest or TA cloning)
  • Flexibility to clone single or multiple fragments—Clone single or multiple DNA fragments simultaneously, in a single reaction
  • Can be used to perform site-directed mutagenesis

Applications:

  • PCR cloning
  • HTP cloning
  • Multiple fragment cloning
  • Gene synthesis
  • Gene design
  • Mutagenesis
  • Domain swapping
  • Domain modification

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