Invitrogen™ Lipofectamine® 2000
The trusted, simple, broad-spectrum transfection reagent for most cell lines
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Great and robust transfection system.
Transfection
We used Lipofectamine 2000 and 3000 and got the same results. They are pretty robust transfection agents in a variety of input nucleic acids. The protocol is easy to understand and use, you get the results within 4 hours.
Review Date: 23 Jul 2021 | Thermo Fisher Scientific
Lipofectamine 2000 achieves 95% transfection efficency.
Lentivirus production
Online instructions were very clear to follow. Good transfection efficiency within 24hr period 95% in HEK293 cells GFP labelled calmodulin construct. Cells seeded at 9x10 6 cells within 10 cm dish.
Review Date: 22 Nov 2019 | Thermo Fisher Scientific
Transfection commonly refers to the introduction of nucleic acids into eukaryotic cells, or more specifically, into animal cells. Invitrogen Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect a variety of payloads into the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 reagent for plasmid DNA transfection as well as siRNA- and shRNA-based gene knockdown experiments and gene expression studies.
Lipofectamine® 2000 reagent delivers DNA or siRNA with excellent transfection performance for protein expression, gene silencing, and functional assays. At the foundation of the popularity of this broad-spectrum reagent is the reliably high efficiency and simple protocol for success across a broad range of cell lines. Lipofectamine® 2000 transfection reagent works effectively with all common cell lines as well as with many challenging ones and can be used in media with or without serum.
Offers:
- Exceptional transfection efficiency in a broad range of cell lines with high levels of recombinant protein expression and a simple protocol
- Superior performance for co-transfection of siRNA and plasmid DNA with reliable performance for high-throughput applications
- Overwhelmingly more publications cite the use of Lipofectamine 2000 Transfection Reagent
