Invitrogen™ Lipofectamine® 3000 Transfection Reagent
Introducing the versatile reagent with the power to transfect your most difficult cells.
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Consistent results and a product of great value
Transfection in hard to transfect cells like AGS and HT-29
The product is very good, using it for the last three years. The transfection efficiency is high and the results are consistent. Some cells are hard to transfect even with this product. Hope to get some updated products from Thermo.
Review Date: 17 Mar 2023 | Thermo Fisher Scientific
Great results.
Pituitary cancer cells transfection
Easy to use and worked well on our primary cells from rats tumor tissues.
Review Date: 17 May 2021 | Thermo Fisher Scientific
First choice for transfection.
In vitro
It is a must for hard-to-transfect cells with more than 80% efficiency.
Review Date: 2 Feb 2021 | Thermo Fisher Scientific
Great efficiency and works consistently.
Transfection into mammalian cells
We use Lipofectamine 3000 to transfect plasmids into both human and mouse cells and receive at least 80-100% efficiency consistently.
Review Date: 17 Apr 2020 | Thermo Fisher Scientific
Transfection efficiency
It would be great if there were more tips & tricks available for optimizing efficiency. Also, it is expensive, but it's still probably the best option.
Review Date: 28 Jan 2020 | Thermo Fisher Scientific
Reliable transfection of siRNA and plasmids.
Over-expression of proteins
Highly effective product. Cost is high and could be more economical since need multiple replicates to perform for publication.
Review Date: 17 Jan 2019 | Thermo Fisher Scientific
Lipofectamine 3000 provides excellent plasmid over-expression, sans cell toxicity.
Over-expression of protein
Lipofectamine 3000 provided gentle but efficient transfection in several line of interest to our lab, HEI-OC1 mouse cochlear (69-fold) , NCCIT and Ntera2 (3 fold) testicular cancer lines and allowed for very nice dose response assays for cisplatin sensitvity assays. We appreciate greatly the technical service of ThermoFisher and find them an integral part of our research progress with excellent technical advise.
Review Date: 5 Nov 2018 | Thermo Fisher Scientific
Great results. Loves to use it.
Mammalian cell transfections
It is extremely easy to use it. And it gives great results.
Review Date: 4 Nov 2017 | Thermo Fisher Scientific
I really enjoy using Lipofectamine reagent for cell transfections
Transfection of cells
In our lab, we use Lipofectmamine 3000 reagent as our primary transfection reagent for a wide range of experiments. I personally have used this reagent to transfect cells for fluorescent microscopy, GST pull downs, and co-immunoprecipitation experiments. We have had success using Lipofectamine reagents in the past and the Lipofectamine 3000 appears to be the best option.
Review Date: 3 Feb 2017 | Thermo Fisher Scientific
Recommend to all researchers for lipid-based reagent for reverse transfection of plasmids
Transfection
Easy protocol, good for reverse transfection with not much toxicity to cells
Review Date: 23 Jun 2016 | Thermo Fisher Scientific
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.
This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
- Superior performance—our highest efficiency reagent for difficult-to-transfect cells.
- Improved cell viability—gentle on your cells, with low toxicity.
- Versatile—one reagent for DNA, RNA, and co-transfection.
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Breast carcinoma (MCF7, Hs578T) Prostate carcinoma (LNCap) Lung carcinoma (A549, NCI-H460) Osteosarcoma (U-2 OS, Saos-2) Colon carcinoma (Caco2, SW480) Pancreatic carcinoma (PANC-1) Skin melanoma (SK-MEL-28)
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Thermo Fisher ScientificContains one vial of Lipofectamine® 3000 Reagent (15 mL) and one vial of P3000™ Enhancer Reagent.