Invitrogen™ Lipofectamine® 3000 Transfection Reagent (1.5 mL)
Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000™ Enhancer Reagent.
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Transfection in hard to transfect cells like AGS and HT-29
The product is very good, using it for the last three years. The transfection efficiency is high and the results are consistent. Some cells are hard to transfect even with this product. Hope to get some updated products from Thermo.
Review Date: 17 Mar 2023 | Thermo Fisher Scientific
Mammalian cell transfections
It is extremely easy to use it. And it gives great results.
Review Date: 4 Nov 2017 | Thermo Fisher Scientific
Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.
This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).
Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:
- Superior performance—our highest efficiency reagent for difficult-to-transfect cells.
- Improved cell viability—gentle on your cells, with low toxicity.
- Versatile—one reagent for DNA, RNA, and co-transfection.
Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:
Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458) Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562) Breast carcinoma (MCF7, Hs578T) Prostate carcinoma (LNCap) Lung carcinoma (A549, NCI-H460) Osteosarcoma (U-2 OS, Saos-2) Colon carcinoma (Caco2, SW480) Pancreatic carcinoma (PANC-1) Skin melanoma (SK-MEL-28)