KAPA DNA Library Preparation Kits for Illumina

KAPA DNA Library Preparation Kits for Illumina sequencing platforms contain evolved and optimally formulated enzymes that enable the highest overall coverage from the least amount of total sequencing.

Kits are optimized to achieve significantly higher library yields and contain KAPA HiFi for high-fidelity, high-efficiency and low-bias library amplification. This ensures the highest library and sequence data quality, particularly from low input and difficult samples such as FFPE and ChIP DNA.

Features:

Achieve greater molecular complexity

• Higher yields of adapter-ligated library translates to higher molecular complexity
• Fewer cycles of amplification are needed, which results in lower PCR duplication rates
• PCR-free workflows possible from inputs ≥100 ng

Reduce amplification bias and improve coverage

• KAPA HiFi reduces PCR bias
• Improves coverage uniformity of GC- and AT-rich regions, promoters, low complexity and other challenging regions

Create high-quality libraries from challenging samples

• High-quality whole exome sequencing from libraries prepared using as little as 10 ng human genomic DNA
• High success rates with 250 ng FFPE or less
• Routine library construction from ≥100 pg ChIP DNA

Automate with pre-validated scripts

• PerkinElmer Sciclone NGS and NGSx
• Beckman Coulter Biomek FXp and FX dual-pod
• Agilent Technologies NGS Workstation Option B
• Eppendorf epMotion 5075 TMX

Applications:

• Whole Genome Sequencing
• ChIP Sequencing
• Whole Exome Sequencing
• Amplicon Sequencing
• Targeted Sequencing (Capture)
• Methyl Sequencing