KAPA HyperPlus Kits
NGS library preparation. Evolved.
KAPA HyperPlus Workflow
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US Pulsenet also using this kit
Library prep
Easy to use
Review Date: 1 Dec 2023 | Kapa Biosystems, Inc.
Efficient and easy to use Illumina library prep kit without need for Covaris
Illumina sequencing
This is an easy to use streamlined kit for preparing Illumina sequencing libraries. One can make libraries in single tube or plates in a little over 2 hours. The chemistry is highly efficient (see Aigrain et al BMC Genomics 2016) allowing for lower input and/or fewer PCR cycles. Enzymatic cleavage appears non-biased.
Review Date: 23 Dec 2016 | Kapa Biosystems, Inc.
The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube.
Building on industry-leading library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows.
Benefits:
- DNA fragmentation and library prep in 2.5 hours
- Flexible DNA sample input from 1 ng – 1 µg
- Reduced bias and maximize sequence coverage
- PCR-free workflows
Features:
Tunable and Reproducible Fragmentation
- Adjust library insert sizes from 150 – 800 bp by varying fragmentation time
- Reproducible insert sizes across a range of GC content and DNA input amounts
Industry Leading Library Yields…
- Routinely achieve conversion rates >80% from ≥ 100 ng input
- Superior performance across a range of DNA input amounts
- High library yields enable PCR-free workflows from as little as 50 ng starting material
…Enable Superior Sequencing Results
- Higher conversion rates results in fewer amplification cycles and lower duplication rates
- Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage
Minimal Sequence Coverage Bias
- Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods
- Equivalent performance to mechanical shearing
- Less bias leads to more uniform sequencing coverage and reduced sequencing costs
Applications:
- Whole Genome Sequencing
- Whole Exome Sequencing
- Targeted Sequencing (custom panels)
- FFPE Sample Sequencing
