KAPA Stranded RNA-Seq with RiboErase
The KAPA Stranded RNA-Seq Kit with RiboErase offers a high-quality, comprehensive solution for transcriptome sequencing. By utilizing a targeted enzymatic method for depletion, our workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPA HiFi for high-efficiency and low-bias library amplifica…
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RNA sequencing library prep
First of all, KAPA's tech support is very useful. Anyone who wishes to use this kit for the first time or not familiar with library prep in general, s/he should definitely consult with them. The protocol is easy to use with multiple safe stopping points and it has a very robust yield (with minimal rRNA for both human and mouse).
Review Date: 5 Apr 2017 | Kapa Biosystems, Inc.
The KAPA Stranded RNA-Seq Kit with RiboErase offers a high-quality, comprehensive solution for transcriptome sequencing. By utilizing a targeted enzymatic method for depletion, our workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPA HiFi for high-efficiency and low-bias library amplification, and include a streamlined, “with-bead” protocol.
- Up to 99.98% rRNA depletion*
- Flexible input of 100 ng – 1 μg total RNA from human, mouse, or rat species*
- Robust and reproducible across various input amounts
- Automation-friendly workflow
For Research Use Only. Not for use in diagnostic procedures.