LDH Assay Kit (Cytotoxicity)
LDH Assay Kit (Cytotoxicity) ab65393 uses WST for the fast and sensitive detection of LDH released from damaged cells.
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Easily used and adaptable to experimental needs
Assay type: Enzyme activity (quantitative)
Very easy to use, extremely fast and sensitive detection of LDH released from damaged cells.
Review Date: 27 Dec 2021 | Abcam plc
Abcam LDH Assay kit.
Cell cytotoxicity
The kit does what it is meant for. The protocol is straightforward and saves a lot of time. I used it for determining the cell cytotoxicity in tissue culture media, basically apical and basal washes from Air liquid Interphase culture. The plate can be read using a normal plate reader.
Review Date: 8 Jun 2021 | Abcam plc
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media
LDH Assay Kit (Cytotoxicity) ab65393 uses WST for the fast and sensitive detection of LDH released from damaged cells.
The LDH assay, also known as LDH release assay, is a cell death / cytotoxicity assay used to assess the level of plasma membrane damage in a cell population. Lactate dehydrogenase (LDH) is a stable enzyme, present in all cell types, which is rapidly released into the cell culture medium upon damage of the plasma membrane. LDH is the most widely used marker used to run a cytotoxicity assay.
The LDH assay protocol is based on an enzymatic coupling reaction: LDH released from the cell oxidizes lactate to generate NADH, which then reacts with WST to generate a yellow color. The intensity of the generated color correlates directly with the number of lyzed cells.
Only 10µl of culture medium is required for the assay, and thus the background from serum and culture medium is significantly reduced. Cells can be cultured in regular 10% serum containing medium; no reducing serum or special medium is required for the assay.
In addition, since WST is very stable, the reaction can be read multiple times and can be stopped at any time point during the reaction.
LDH activity can be easily quantified by spectrophotometer or plate reader at OD450nm.
LDH assay protocol summary:
- transfer 10µl culture medium into new plate
- add LDH reaction mix and incubate for 30 min at room temp
- analyze with microplate reader
