Magic Red™ Caspase 3 & 7 Assay Kit
Magic Red™ Caspase 3 and 7 Assay is a substrate-based fluorescent apoptosis assay that measures DEVDase enzyme activity in living, intact cells. Detection of caspase 3 and 7 activity (DEVDase enzymes) is a reliable method for assessing apoptotic activity in experimental cell populations. The Magic Red™ caspase 3 and 7 fluorogenic substrate, MR-(DEVD)2, is primarily targeted by caspases 3 and 7 of the apoptotic protease casca…
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Magic Red™ Caspase 3 and 7 Assay is a substrate-based fluorescent apoptosis assay that measures DEVDase enzyme activity in living, intact cells. Detection of caspase 3 and 7 activity (DEVDase enzymes) is a reliable method for assessing apoptotic activity in experimental cell populations.
The Magic Red™ caspase 3 and 7 fluorogenic substrate, MR-(DEVD)2, is primarily targeted by caspases 3 and 7 of the apoptotic protease cascade. The presence of the active form of caspases 3 and 7 in apoptotic cells causes the hydrolysis of the two DEVD target sequences from the cresyl violet fluorophore (Magic Red™), converting it to the fluorescent form. When the cresyl violet dye is di-substituted with two DEVD caspase-3/7 target sequences, the reagent is essentially non-fluorescent. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases.
Kit Specs:
- Contents: Magic Red™-(DEVD)2 substrate reagent, Hoechst 33342 stain, and acridine orange stain.
- Excitation of Magic Red™: 540-590 nm (590 nm optimal)
- Emission of Magic Red™: >610nm (630 nm optimal)
- Cell membrane permeant reagents; no lysis
- Substrate-based assay; no wash step
- Analysis via fluorescence microscopy,fluorescence plate reader, or flow cytometer
