MeDIP Kit
Immunoprecipitate and enrich for single-stranded DNA fragments containing 5-methylcytosine (5-mC)
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Methylated DNA Immunoprecipitation (MeDIP) is an immunocapture technique in which an antibody specific for methylated cytosines is used to immunoprecipitate methylated genomic DNA fragments.
MeDIP uses single-stranded DNA that has been fragmented by sonication or enzymatic digestion. The addition of a monoclonal antibody specific for 5-methylcytosine in the presence of a bridging antibody enables capture of the DNA fragments containg 5-mC methylation. The use of Protein G magentic beads provides quick and efficient capture. Methylated fragments are then eluted from the magnetic beads providing DNA samples that are specifically enriched for 5-mC DNA methylation. This selectivity is important as most common approaches to analyze DNA methylation, such as enzymatic approaches and bisulfite conversion, are unable to distinguish between 5-mC and 5-hmC.
