MegaMix HRM
MegaMix HRM simplifies HRM-PCR with its 2X mix featuring chemically modified Hot Start Taq polymerase and microGREEN dye in an optimized buffer for HRM analysis. The polymerase is inactive until 95°C, ensuring high sensitivity and specificity, preventing non-specific amplification. Ideal for GC-rich templates, it delivers reproducible results with minimal optimization.
Receive your quote directly from the manufacturer.
HRM is a quick and simple process that allows detection of variations in DNA sequences. It enables the detection of single nucleotide polymorphisms by distinguishing minor differences in melting curves.
MegaMix HRM contains all the components needed to perform HRM-PCR swiftly and reliably. The 2X mix contains chemically modified Hot Start Taq DNA polymerase and microGREEN intercalating dye in enhancing buffer optimised for HRM analysis. microGREEN is a third-generation, saturating, intercalating fluorescent dye that binds to double stranded DNA without inhibiting PCR; making MegaMix HRM the perfect choice for HRM.
The Hot Start Taq polymerase is chemically inactivated until heating to 95°C, providing excellent sensitivity and specificity, eliminating the formation of non-specific amplification and primer-dimers.
This mastermix performs excellently when amplifying from GC-rich templates, proving reproducible and reliable results with little or no optimisation.
Key Features
- Save Time and Money—quickly and efficiently identify sequence variations.
- Specificity—Hot Start Taq DNA Polymerase in optimised buffer eliminates non-specific amplification and the formation of primer dimers.
- Sensitivity—detect class 4 SNP.
- Versatile— compatible with standard and fast cycling conditions, GC/AT rich templates.
- Reproducibility and convenience—ready to use 2X format.
- Third-generation intercalating dye— no inhibition of PCR, even at high concentrations, suitable for High Resolution Melting.
Applications
High Resolution Melt (HRM) analysis:
- Single Nucleotide Polymorphism (SNP) genotyping.
- Methylation analysis.
- Mutation scanning.
- Detection of sequence variations.
