MegaMix Royal
MegaMix Royal features Hot Start Taq DNA polymerase, 400μM dNTP, 6 mM MgCl2, and blue loading dye for precise PCR. Its superior hot start polymerase at 95°C ensures specific amplification, while providing accurate A-tailed products suitable for TA cloning. The blue agarose dye aids visualization without affecting downstream processes.
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Containing all the components needed to perform PCR swiftly and reliably.
The 2X mix contains Hot Start Taq DNA polymerase, 400μM dNTP, 6 mM MgCl₂ and blue loading dye in Microzone's proprietary enhancing buffer. MegaMix Royal uses a superior sensitive hot start DNA polymerase.
The polymerase becomes active upon heating at 95°C. This ensures a highly specific and sensitive amplification, removing background and primer dimer formation.
MegaMix Royal boasts excellent accuracy and produces A-tailed products suitable for ligating into TA cloning vectors. The blue agarose loading dye incorporated allows easy visualisation and eliminates the need for additional gel loading buffers. The dye does not inhibit restriction enzymes or ligases and does not fluoresce at the wavelengths used by automated DNA sequencers so downstream processes are not impacted.
Key Features
- Hot Start polymerase in Microzone’s proprietary buffer gives unrivalled confidence in PCR amplifications.
- 2X concentrated format.
- Broad range of templates and conditions.
- Extremely stable—can be freeze-thawed many times.
- Easy setup and PCR optimisation.
Applications
- Hot Start PCR up to 6 kb.
- qPCR (probe or dye based).
- Fast PCR.
- Multiplex PCR.
- Genotyping.
- Amplification of GC and AT rich templates.
- TA Cloning.
- High Resolution Melting.
