Megaruptor®
Diagenode’s ultimate solution for long fragment library preparation between 2kb and 75kb– the Megaruptor® fragmentation system. A number of applications for massively parallel sequencing, also known as Next-Generation Sequencing, require libraries of long DNA segments, but few methods have been available for reliably generating fragments greater than 2 kb. The Megaruptor, which has been used by several sequencing centers w…
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absolutely necessary for long read sequencing applications
DNA fragmentation
Absolutely critical for our long read sequencing projects. It's the most effective tool for fragmenting DNA to an appropriate range for projects using high molecular weight DNA such as PacBio and Oxford Nanopore. It's reliable, consistent, and effective
Review Date: 28 Jun 2023 | Diagenode
Diagenode’s ultimate solution for long fragment library preparation between 2kb and 75kb– the Megaruptor® fragmentation system. A number of applications for massively parallel sequencing, also known as Next-Generation Sequencing, require libraries of long DNA segments, but few methods have been available for reliably generating fragments greater than 2 kb.
The Megaruptor, which has been used by several sequencing centers worldwide to produce insert fragment libraries for Illumina, Ion Torrent, and PacBio platforms, utilizes mechanical shearing to generate libraries between 2 kb and 75 kb.
The instrument’s unique feature is the “Hydropore” shearing device that eliminates clogging issues frequently encountered with single orifice shearing devices and includes a disposable element that reduces the potential for cross-contamination.
A common application that requires long insert paired-end DNA libraries is mate pair library preparation, useful for de novo sequencing, detecting structural variants, and identifying rearrangements, duplications, and repeats in the genome.
Features & Benefits:
- Achieve a tight distribution of fragment lengths, easily tuneable to between 2 kb and 75 kb
- Obtain high-quality libraries for Illumina®, Ion Torrent™, and PacBio® platforms
- Process 2 samples in series with walk-away capability
- Alleviate clogging issues typically encountered with single-orifice devices thanks to innovating technology
- Eliminate cross-contamination with disposable element
- Cost-effective solution
