MethylCollector™ Ultra
Fast magnetic assay capable of efficiently isolating methylated CpG islands from fragmented genomic DNA
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Active Motif's MethylCollector™ Ultra Kit is an improved alternative for the enrichment of CpG-methylated DNA from limited amounts of cell or tissue samples.
The method is based on the Methylated CpG Island Recovery Assay (MIRA) which utilize the high affinity of the MBD2b/MBD3L1 complex for methylated DNA. MethylCollector Ultra is suitable for use in many downstream applications, such as real time or endpoint PCR analysis of the methylation status of particular loci in normal and diseased samples, rapid screening of the methylation status of multiple loci, bisulfite conversion followed by cloning and sequencing, or amplificaiton and labeling for microarray analysis. It can also be used to detect changes in DNA methylation during normal cellular differentiation and aging.
