MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit
For quantitation of global DNA methylation via a one-step ELISA method
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Quick and easy but not reliable!
Anayze DNA methylation percentage from mammalian DNA samples
We bought this product for use in quantifying DNA methylation from Rat brain samples. It has been used in multiple publications however, while the product was easy to use it lacked reproducibility and robustness. There was high intra and inter assay variability and we lacked confidence in the product output. Technical support from Epigentek was good and fast but the problems could not be resolved and we transferred to another kit for our samples.
Review Date: 6 Nov 2019 | Epigentek
Manufacturer's Response
Thank you for using EpiGentek’s products, and we are sorry about the issue you have had with the MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit. Unfortunately, we were unable to verify when the kit was purchased or what the specific issue was, as we do not have a record of the technical support inquiry. We would be more than happy to learn more about the problem and help troubleshoot the issue. We would also gladly consider a possible concession based upon your feedback. Thank you again.
The MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit is a complete set of optimized buffers and reagents to colorimetrically quantify global DNA methylation status by specifically measuring levels of 5-methylcytosine (5-mC) in a simplified, "one-step" ELISA-like reaction. This allows global DNA methylation to be directly measured more quickly than other techniques such as LUMA, LINE-1, Alu or LTR-based assays.
As a fourth generation technology of EpiGentek's patented global DNA methylation technique, it is a further refinement of the popular, predecessor MethylFlash kit by improving upon speed, simplicity, sensitivity, and reproducibility.
This kit is also specifically optimized for paired use with the MethylFlash Global DNA Hydroxymethylation (5-hmC) ELISA Easy Kit (Colorimetric) for simultaneously quantifying both methylated DNA and hydroxymethylated DNA.
This kit has the following advantages and features:
- Fast - Reduced steps so that the entire procedure only needs 2 hours
- Robust - Improved kit composition allows the assay to have a greater "signal window" with reduced variation between replicates
- Convenient - Inherently low background noise, thereby eliminating the need for DNA denaturation and plate blocking steps
- Sensitive - Detection limit can be as low as 0.05% methylated DNA from 100 ng of input DNA
- Specific - High specificity to 5-mC, with no cross-reactivity to unmethylated cytosine and no cross-reactivity to hydroxymethylcytosine within the indicated concentration range of the sample DNA
- Universal - Positive and negative controls are included and allow for detection of DNA methylation in any species from either single-stranded or double-stranded input DNA
- Accurate - Optimized positive controls that can be fractionalized in percentage scale, allowing the assay to be more accurate and highly comparable with HPLC-MS analysis
- Flexible - Strip-well microplate format makes the assay available for manual or high throughput analysis
