NASBA Lyophilized Kit (10 reactions)
Easily amplify and detect RNA with our NASBA kits
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Reverse transcriptase PCR (RT-PCR) is currently one of the most commonly used techniques for performing RNA detection, although it suffers from false positives due to cross-contamination whilst being a time consuming process. A more convenient technique for RNA detection is Nucleic Acid Sequence Based Amplification (NASBA), which is a one-step isothermal process for amplifying RNA. NASBA has demonstrated success in the detection of viral (including SARS-CoV-2) and bacterial RNA from clinical samples. Each NASBA reaction contains avian myeloblastosis virus-reverse transcriptase (AMV-RT), T7 RNA polymerase and RNase H with reaction buffer. Due to NASBA being an isothermal process, denaturation of dsDNA does not occur and ssRNA can be amplified. Therefore, false negatives for the NASBA reaction are prevented that would otherwise be caused by genomic dsDNA in other techniques like RT-PCR. NASBA lyophilized kit key features and details
- Contains all the components needed to perform NASBA reactions
- Higher amplification kinetics compared to RT-PCR
- Cost-effective - no need for thermocycling equipment
- Suitable for human mRNA detection without risking DNA contamination
- Amplify nucleic acid sequences in less than 90 minutes
- Suitable for use with COVID-19 samples
