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Normal Serums & Gamma Globulins

Normal serum can be used as a blocking agent to reduce background. Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding. Best results are obtained with diluted nor…

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Jackson ImmunoResearch Laboratories Inc.

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Average Rating 4.5

|2Scientists have reviewed this product

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Great blocking

 

Average Rating 4.7

Application Area:

Immunohistochemistry

Effective blocking for immunochemical assays, especially for IHC and ICC, to reduce background.

Review Date: 22 Jan 2018 | Jackson ImmunoResearch Laboratories Inc.

Very good results, I recommend it

 

Average Rating 4.3

Application Area:

Analyse metabolites in tissue samples

Reproducible results very good. Quality of the product very good. Easy to use. Money: it is a bit pricey but I can t complain. I also recommended it to other labs in our department.

Review Date: 8 Aug 2016 | Jackson ImmunoResearch Laboratories Inc.

Normal serum can be used as a blocking agent to reduce background.

Normal serums are lipid extracted to improve clarity, dialyzed against phosphate buffered saline (PBS) containing sodium azide, and freeze-dried. Normal serum diluted to 5% (v/v) in PBS is strongly recommended as a blocking agent to reduce background from non-specific, conserved-sequence, and/or Fc-receptor binding. Best results are obtained with diluted normal serum from the same host as the labeled antibody, as a separate incubation step before addition of the primary antibody.

Gamma globulins are further purified from non-immunized animal serums by salt fractionation, ion-exchange chromatography, and gel filtration. Gamma globulins are an inexpensive source of IgG with only trace amounts of other immunoglobulins and/or non-immunoglobulin serum proteins. Gamma globulins are supplied as sterile liquids in phosphate buffer without stabilizers or preservatives.

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