Nucleofector® II Device
The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format. What benefits are important for your work? Efficient transfection in single cuvette format? Up to 90% efficiency with plasmid DNA…
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Nucleofector® II is a very good product
Transfection
This system works exactly as intended. Nucleofections are easier to perform as well as scalable to low and high throughput. Great service and wide selection of protocols available with minimal troubleshooting and not very expensive.
Review Date: 8 Jul 2022 | Lonza Group Ltd
Great system for nuceofecting!
Transfection
This system works exactly as intended. Nucleofections are easier to perform as well as scalable to low and high throughput. Great service and wide selection of protocols available with minimal troubleshooting.
Review Date: 6 Feb 2019 | Lonza Group Ltd
Great method for getting constructs and siRNA into cell lines
Transfection of cell lines
This is a great method for very high efficiency of cultured cell lines. It's simple to use, very highly reproducible.
Review Date: 9 Sept 2016 | Lonza Group Ltd
Nucleofection Of Primary Cortical Neurones (E16)
I use the lonza kits (cuvettes) and provided buffer. On the day of plating I get over 80% nucleofection in general and minimal cell death. I've used this product to nucleofect primary cortical, striatal, hippocampal and mixed cultures with great success.
Review Date: 29 Nov 2013 | Lonza Group Ltd
mice hippocampal neuron
I used this equipment to study the mophology of neuronal cytoskelon in cell line induced neurons and primary culture hippocampal neurons and cortical neurons.
Review Date: 16 Dec 2012 | Lonza Group Ltd
There are new versions available, but we are concerned about reproducibility if we switch.
Review Date: 28 Jun 2011 | Lonza Group Ltd
The basic Nucleofector® II Device is a single cuvette based system that has been used in research labs since 2001. It allows efficient transfection of hard-to-transfect cell lines and primary cells with different substrates, e.g. DNA vectors or siRNA oligonucleotides, in low throughput format.
What benefits are important for your work?
- Efficient transfection in single cuvette format?
- Up to 90% efficiency with plasmid DNA
- Up to 99% efficiency with siRNA duplexes
- Also suited for peptides, proteins or small molecules
Reliable easy-to-use technology?
- More than 150 ready-to-use Amaxa® Optimized Protocols
- High viability and preservation of cell funcionality
- Data for more than 650 cell types in our Cell Database
- Consumables tailored to your needs?
- Nucleofector® Kits are available in low (10 reactions), normal (25 reactions) and high (100 reactions) usage format
- More than 50 dedicated primary cell kits available
- A collection of 5 cell line kits and an optimization kit covering the broadest range of cell lines
cGMP kits available
