PlexFlo
Bruker microfluidics accessory units are designed to allow sequential labeling to be incorporated into localization microscopy protocols with the Vutara VXL single-molecule localization microscope. Bruker offers two units—PlexFlo and PlexFlo96. The units are controlled by Vutara's SRX software and allow flexible configuration of fluidics sequences that are run in conjunction with imaging cycles to allow users to label an unli…
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Accessory for sequential labeling in localization microscopy applications --- Bruker’s PlexFlo Multiplexing Platform enables impressive multiplexing capabilities with ease where buffer reservoirs can be swapped out during experiments. Bruker’s PlexFlo and PlexFlo96 are controlled by Vutara’s SRX software and allow flexible configurations of fluidics sequences that are run in conjunction with imaging cycles to allow users to label an unlimited number of probes before imaging them in a sequential fashion. This makes these systems ideal for developing applications in localization microscopy where the number of targets for imaging far outstrips the number of probes that can be chromatically separated. Additionally, these systems’ distinct features make them optimal for various super-resolution techniques such as DNA-PAINT, OligoSTORM, multiplexed antibody labeling, and chromatin tracing. Additionally, each system provides pressure-driven flow control, software integration, and an automatic cleaning procedure. PlexFlo96 expands on these capabilities by offering 96 buffer reservoirs (compared to 15) and sample temperature control (39°- 99° F; 4°- 37°C in a microwell plate).
