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prepGEM Bacteria

prep GEM Bacteria is ideal for processing Gram-positive bacteria, Gram-negative bacteria, protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water. The heat-driven extraction is done in a single-tube with no harsh chemicals or additional steps so there is no loss of DNA, enabling extractions from very low sample volume.

MicroGEM

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MicroGEM’s Bacteria kit provides a simplified approach to studying a wide range of microbial cultures. Every aspect of the MicroGEM method ensures the samples are processed quickly and gently. Minimal sample prep, closed tube processing, and gentle lysis ensure that cross contamination and DNA fragmentation are drastically reduced, making MicroGEM’s heat-driven, single-tube extraction process streamlined while preserving the integrity of low abundance species.

The process utilizes a thermophilic proteinase activated with a simple temperature change. The whole process is easily automated in a single tube using a standard thermocycler.

  • Novel temperature controlled enzymatic lysis using a thermophilic proteinase in combination with mesophilic Lysozyme
  • Rapid workflow- 1/4th the time taken by other methods
  • Easily automated for high throughput
  • Closed system- no cross contamination and protection against pathogens
  • Gentle lysis with no transfer steps- preserves DNA and improves community representation in metagenomic samples
  • Minimal handling and pipetting of samples- DNA is exceptionally intact and high molecular weight – perfect for nanopore sequencing
  • Can be easily adapted to different sample types and workflows

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