Rat TNF alpha ELISA Kit (ab46070)
Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples. A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wel…
The supplier does not provide quotations for this product through SelectScience. You can search for similar products in our Product Directory.
Reasonable price and good quality
analyze quantity of TNF alpha in tissue homogenate
One of the key advantages of the ABCAM TNF alpha ELISA kit is its high sensitivity and specificity, which allows for the detection of even low levels of TNF alpha in biological samples. The kit also offers a broad dynamic range, which enables the detection of a wide range of TNF alpha concentrations, thereby increasing its versatility and applicability in a variety of research settings. the ABCAM TNF alpha ELISA kit is a reliable and effective research tool for the detection and quantification of TNF alpha protein levels in biological samples. Its high sensitivity, specificity, and reproducibility, combined with its versatility and customizable options, make it a valuable asset for researchers in the fields of immunology, inflammation, and related areas of study.
Review Date: 24 Jul 2023 | Abcam plc
Great cytokine quantification
Analyze cytokine in cultures medium
Good product for the quantification of cytokines in culture media of organotypic hippocampal slices. The kit works with both for low and high concentrations. The protocol is clearly described and easy to use.
Review Date: 26 Mar 2018 | Abcam plc
Abcam’s TNF alpha Rat in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Rat TNF alpha in cell culture supernatants, serum and plasma samples.
A monoclonal antibody specific for TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples.
This kit will recognize both endogenous and recombinant rat TNF alpha.
- Sample type: Cell culture supernatant, Serum
- Assay type: Sandwich (quantitative)
- Sensitivity: < 15 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Assay time: 3h 45m
- Assay duration: Multiple steps standard assay
- Species reactivity: Rat
